Cleaved Amplified Polymorphic Sequences (CAPS) Markers for Characterization of the LuFAD3A Gene from Various Flax (Linum usitatissimum L.) Cultivars

Depending on the variety, flax (Linum usitatissimum L.) provides an oil rich in omega-3 acids (especially 50% ?-linolenic acid) with proven health properties, and is used as a raw industrial material. Alpha-linolenic acid polyunsaturated fatty easily subject to oxidative transformation. The auto-oxidation of main process contributing off-flavor, color loss, change nutritional value oil. We six genotypes differing content our research. For all DNA samples extracted from leaf tissue studied flax, new, cleaved amplified polymorphic sequences (CAPS) markers specific LuFAD3A desaturase gene were applied. A PCR product comprising fragments exon 5, 6, intron between these exons was digested using various restriction enzymes. These experiments could differentiate some varieties flax. also proved that mutation previously detected by other authors low-linolenic forms (solin line 593–708) absent we (Linola KLA Linola KLB).